Studies on reducing of phosphorus and nitrogen excretion in Yellowtail (Seriola quinqueradiata) nutrition: I. Effects of dietary protein and energy level

Phosphorus and nitrogen excretion in Yellowtail




Phosphorus, nitrogen, excretion, dietary protein, lipid, yellowtail


The objective of the present study was to investigate the effect of increasing dietary energy at varying dietary protein levels on reducing phosphorus and nitrogen excretion in young yellowtail (Seriola quinqueradiata) in a 55-day growth trial by using 180g of fish. Three levels of dietary protein (46%, 50% and 54%) and two levels of dietary energy were tested in a complete randomized design with a 3 x 2 factorial arrangement. Dietary protein and energy levels did not cause any significant difference in final weight, weight gain, feed intake or survival. However, feed and protein efficiencies significantly (P < 0.05) improved with increasing dietary energy in each respective dietary protein levels.  Phosphorus and nitrogen excretion rates were significantly (P < 0.05) reduced with increasing dietary energy at all dietary protein level. There was no interaction between dietary protein and energy level on the parameters measured.  In conclusion, the diet containing 46% protein and 19.5% lipid with the total energy content of 5.2 kcal / g was recommended for yellowtail growing from 180 g to 460 g to obtain the lowest phosphorus and nitrogen excretion values without reduction of growth performances under the present experimental conditions.


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A.O.A.C. (1990). Official Methods of Analysis of the Association of Official Analytical Chemists, 15th ed. Association of Official Analytical Chemists, Arlington, VA, USA.

Arzel, J., Martinez Lopez, F. S., Metailler, R., Stephan, G., Viau, M., Gandemer, G., & Guillaume, J. (1994). Effect of dietary lipid on growth performances and body composition of brown trout (Salmo trutta) reared in seawater. Aquaculture, 123, 361-375.

Bureau, D. P., & Cho, C. Y. (1999). Phosphorus utilization by rainbow trout (Oncorhynchus mykiss): estimation of dissolved phosphorus waste output. Aquaculture, 179, 127–140.

Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physioogy, 37, 911–917.

Chan, J. C. K., Mann, M., Skura, B. J., Rowshandeli, M., Rowshandeli, N., & Higgs, D. A. (2002). Effects of feeding diets containing various dietary protein and lipid ratios on the growth performances and pigmentation of post-juvenile coho salmon Oncorhynchus kisutch reared in sea water. Aquaculture Research, 33, 1137-1156.

Cho, C. Y, Hynes, J. D, Wood, K. R., & Yoshida, H. K. (1994). Development of high-nutrient-dense, low-pollution diets and prediction of aquaculture waste using biological approaches. Aquaculture, 124, 293–305.

Kaushik, S. J., & Medale F. (1994). Energy requirements, utilization and dietary supply to salmonids. Aquaculture, 124, 81-97.

Kim, K. I., & Kaushik, S. J. (1992). Contribution of digestible energy from carbohydrates and estimation of protein/energy requirements for growth of rainbow trout (Oncorhynchus mykiss). Aquaculture, 106, 161-169.

Lall, S. P. (1991). Digestibility, metabolism and excretion of dietary phosphorous in fish. In: Cowey, C. B., & Cho, C. Y. (Eds.), Nutritional Strategies and Aquaculture Wastes, University of Guelph, Fish Nutrition Research Laboratory, University of Guelph, Guelph, Ontario, Canada, N1G 2W1, pp. 21–36

Lowry, O. H., & Lopez, J. A. (1946). The determination of inorganic phosphate in the presence of labile phosphate esters. Journal of Biological Chemistry, 62, 421–428.

NRC (National Research, Council), (1993). Nutrient Requirements of Fish. National Academy Press, Washington, DC, USA. 114 pp.

Ren, T., Teshima, S., Panganiban JR, A., Uyan, O., & Alam, Md. S. (2008). Effectivenes of L- ascorbyl-2-monophosphate Na/Ca as a vitamin C source for yellowtail Seriola quinqueradiata juveniles. Aquaculture Nutrition, 14, 416-422.

Roy, P. K., & Lall, S. (2003). Dietary phosphorus requirement of juvenile haddock (Melanogrammus aeglefinus L.). Aquaculture, 221, 451–468.

Satoh, S., Sarker, S. A., Satoh, K., & Kiron, V. (2004). Effects of dietary lipid and phosphorus levels on nitrogen and phosphorus excretion in young yellowtail Seriola quinqueradiata: A preliminary observation. Fisheries Science, 70, 1082–1088.

Steffens, W., Rennert, B., Wirth, M., & Kruger, R. (1999). Effect of two lipid levels on growth, feed utilization, body composition and biochemical parameters of rainbow trout, Oncorhynchus mykiss (Walbaum 1792). Journal of Applied Ichthyology, 15, 159–164.

Uyan, O., Koshio, S., Ishikawa, M., & Yokoyama, S. (2023). Effect of fish size on nitrogen utilization in Japanese flounder, Paralichthys olivaceus (Temminck & Schlegel). Marine Reports, 2(2), 105-117.

Uyan, O., Koshio, S., Ishikawa, M., Yokoyama, S., Uyan, S., Ren, T., & Hernandez, L. H. (2009). The influence of dietary phospholipid level on the performances of juvenile amberjack, Seriola dumerili, fed non-fishmeal diets. Aquaculture Nutrition, 15, 550 - 557.

Weatherup, R. N., McCracken, K. J., Foy, R., Rice, D., McKendry, J., Mairs, R. J., & Hoey, R. (1997). The effects of dietary fat content on performance and body composition of farmed rainbow trout (Oncorhynchus mykiss). Aquaculture, 151, 173–184.




How to Cite

UYAN, O., KOSHIO, S., ISHIKAWA, M., & YOKOYAMA, S. . (2024). Studies on reducing of phosphorus and nitrogen excretion in Yellowtail (Seriola quinqueradiata) nutrition: I. Effects of dietary protein and energy level: Phosphorus and nitrogen excretion in Yellowtail. MARINE REPORTS (MAREP), 3(1), 48–55.



Research Article