Effects of feeding frequency on growth performances and nutrient digestibility of juvenile Japanese flounder, Paralichthys olivaceus (Temminck & Schlegel), under varied dietary protein levels

Feeding frequency in Japanese flounder


  • Orhan UYAN
  • Shunsuke KOSHIO
  • Manabu ISHIKAWA
  • Saichiro YOKOYAMA




Feeding frequency, dietary protein, digestibility, Japanese flounder, Paralichthys olivaceus


The effects of feeding frequency on growth, body composition and nutrient digestibility were studied for the juveniles of Japanese flounders (Paralichthys olivaceus) under four different dietary protein levels. Four isocaloric fishmeal-casein based diets containing different dietary protein levels from 46 to 65% were formulated and fed to juvenile flounder (initial mean weight 0.4 g) daily near satiation corresponding to three different feeding frequencies (1, 2 and 3 times/day) for 40 days. Results of feeding trial indicated that both dietary protein level and feeding frequency significantly affected the growth parameters. Regardless of the dietary protein level, the optimum growth performance was obtained from the fish fed 2 times/day. Under two times feeding a day, no significant difference was found between the fish fed diet containing 54 and 59% protein, and no further significant improvement on growth was observed when the dietary protein level increased from 59 to 65%. Protein digestibility was not affected by the feeding frequency or dietary protein level. Findings of this study suggest that feeding frequency twice per day was found to be the optimal, and the optimum dietary protein level could be 54-59% for the superior growth performances of juvenile Japanese flounder from 0.4 to 18 g.


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How to Cite

UYAN, O., KOSHIO, S., ISHIKAWA, M., & YOKOYAMA, S. (2022). Effects of feeding frequency on growth performances and nutrient digestibility of juvenile Japanese flounder, Paralichthys olivaceus (Temminck & Schlegel), under varied dietary protein levels: Feeding frequency in Japanese flounder. MARINE REPORTS (MAREP), 1(2), 110–121. https://doi.org/10.5281/zenodo.7394102



Research Article