Investigation of the Isolation Streptomyces from Marine Mucilage

Abstract

This study aims to isolate of Streptomyces from mucilage samples collected from the Çanakkale Strait (Dardanelles). Mucilage is a highly viscous structure produced by various microorganisms (diatoms, dinoflagellates, cyanobacteria, and bacteria) under suitable environmental conditions. Although the literature states that mucilage may contain different microorganisms, no studies have specifically examined Streptomyces species within mucilage structures. This study cultivated Streptomyces species isolated from mucilage in three different growth media (GYM-Streptomyces, N-Z-Amine, and ISP 4) with and without calcium carbonate (CaCO₃) pretreatment. The results showed that ISP 4 was the most effective medium for Streptomyces growth, while CaCO₃ pretreatment significantly enhanced Streptomyces isolation efficiency by reducing the total bacterial load. 118 Streptomyces isolates were obtained, with 62 isolates from ISP 4, 36 from N-Z-Amine, and 20 from GYM. After CaCO₃ pretreatment, the total number of Streptomyces isolates increased approximately 3.5 times (from 26 to 92) in all media. This suggests that CaCO₃ effectively enhances Streptomyces recovery from mucilage samples.

This study demonstrated that Streptomyces species can be successfully isolated from mucilage structures, and further research should explore their potential applications in biotechnology, particularly in antimicrobial and pharmaceutical studies